ABSTRACT
We studied the hydrogen evolution (HE) of green alga Chlorella pyrenoidosa grown in normal (nutrients sufficient) media and nitrogen, manganese or sulfur deprived medium. The results showed that photo-hydrogen evolution could occur under all conditions herein before, but the efficiency of HE was maximum under nitrogen deprivation, and the total hydrogen yield was 88.613 microL H2/mg Chla, which was 4.61, 1.92, 3.63 times of control, manganese deprivation, sulfur deprivation groups, respectively. We also measured the growth, the photosynthesis and respiration of the alga. The data demonstrated that manganese deprivation had less influence than nitrogen and sulfur deprivation on the growth, the photosynthesis and respiration of C. pyrenoidosa. Compared with the normal (nutrients sufficient), manganese and sulfur deprivation inhibited the photosynthesis and growth of the alga while bringing small impact on respiration. Nitrogen deprivation, however, greatly restrained the photosynthesis and growth while enhancing the respiration. Those data provide clues for the further study on both the conditions optimization and mechanism of hydrogen evolution.
Subject(s)
Chlorella , Metabolism , Hydrogen , Metabolism , Manganese , Metabolism , Nitrogen , Metabolism , Photosynthesis , Sulfur , MetabolismABSTRACT
From 6 stains of yeasts, Saccharomyces cerevisiae 2016 which was suitable for Cr rich yeasts fermentation was selected Concentration and additive method of CrCl 3 were investigated by liquid culture and the optimum conditions of Cr rich yeasts were obtained The optimum concentration of CrCl 3 were 5?10 4 mol/L that were added to liquid medium at the initial 8 h of culture time At this condition, the biomass of Cr rich yeasts was 1 55 g dry weight per 100 milliliter, Cr content in yeast cells was above 1100 ?g/g, the utilizable rate of Cr ion was about 60%